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Visualisere Multiciliated celler i zebrafisk gennem en kombineret protokol af hele Mount fluorescerende In Situ hybridisering og immunfluorescens
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

Visualisere Multiciliated celler i zebrafisk gennem en kombineret protokol af hele Mount fluorescerende In Situ hybridisering og immunfluorescens

DOI:
10.3791/56261-v

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09:33 min

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November 18, 2017

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, , , ,
1Department of Biological Sciences,University of Notre Dame

Chapters

  • 00:05Title
  • 00:50Embryo Fixation and Hybridization
  • 03:05Hot Washes, Blocking, Antibody Incubation, and Maleic Acid Buffer Washes
  • 04:27Probe Detection, Peroxidase Removal, and Immunofluorescence
  • 07:19Zebrafish Pronephros Mounting and Imaging
  • 08:23Results: Representative Zebrafish Pronephros and Multiciliated Cell (MCC) Imaging
  • 09:09Conclusion

Summary

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自动翻译

Cilier udvikling er afgørende for korrekt organogenesis. Denne protokol beskriver en optimeret metode til at mærke og visualisere ciliated celler af zebrafisk.

Tags

Keywords: Multiciliated CellsZebrafishWhole Mount Fluorescent In Situ HybridizationImmunofluorescence发育生物学PronephrosCiliaRNA TranscriptsProtein LabelingAntibodiesFixationRehydrationProteinase KHybridization SolutionRNA Probe

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