Journal
/
发育生物学
/
全贴装荧光原位杂交和免疫荧光法在斑马鱼中 Multiciliated 细胞的可视化
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
Please note that all translations are automatically generated. Click here for the English version.

全贴装荧光原位杂交和免疫荧光法在斑马鱼中 Multiciliated 细胞的可视化

DOI:
10.3791/56261-v

•

09:33 min

•

November 18, 2017

•

, , , ,
1Department of Biological Sciences,University of Notre Dame

Chapters

  • 00:05Title
  • 00:50Embryo Fixation and Hybridization
  • 03:05Hot Washes, Blocking, Antibody Incubation, and Maleic Acid Buffer Washes
  • 04:27Probe Detection, Peroxidase Removal, and Immunofluorescence
  • 07:19Zebrafish Pronephros Mounting and Imaging
  • 08:23Results: Representative Zebrafish Pronephros and Multiciliated Cell (MCC) Imaging
  • 09:09Conclusion

Summary

自动翻译

自动翻译

纤毛发育对正常器官的形成至关重要。该协议描述了一种优化的方法来标记和可视化纤毛细胞的斑马鱼。

Tags

Keywords: Multiciliated CellsZebrafishWhole Mount Fluorescent In Situ HybridizationImmunofluorescence发育生物学PronephrosCiliaRNA TranscriptsProtein LabelingAntibodiesFixationRehydrationProteinase KHybridization SolutionRNA Probe

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/56261?article_type=v

了解早期器官使用简体<em>原位</em>杂交协议<em>爪</em
check_url/cn/56261?article_type=v

多目标显色全安装<em>原位</em>在比较基因表达域杂交<em>果蝇</em>胚胎
check_url/cn/56261?article_type=v

方法来检查淋巴腺和血细胞中<em>果蝇</em>幼虫
check_url/cn/56261?article_type=v

器官培养和鼠标沃尔弗导管的全山免疫染色
check_url/cn/56261?article_type=v

水平整体安装：厚三维组织横截面皮肤的新型加工和成像方案
check_url/cn/56261?article_type=v

小鼠胚胎心肌内祖群的定量全贴免疫荧光分析
check_url/cn/56261?article_type=v

培养小鼠卵巢的全贴装免疫荧光和卵泡定量研究
check_url/cn/56261?article_type=v

用扫描电镜和全贴免疫荧光技术可视化小鼠胚胎中的节点和外型板
check_url/cn/56261?article_type=v

斑马鱼原位杂交染色的基因分型与定量
check_url/cn/56261?article_type=v

斑马鱼胚胎全山原位杂交和管状测定在iPSC-ECs中研究恩多林在血管发育中的作用

Read Article