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A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies
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生物化学
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JoVE 杂志 生物化学
A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies

A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies

11,072 Views

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10:47 min

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July 24, 2017

DOI:

10.3791/56080-v

DOI:
10.3791/56080-v

•

10:47 min
July 24, 2017

•

3 Views
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1Department of Neurology,University of Southern California, 2Section of Cell and Developmental Biology,University of California San Diego

Tags

Yeast-one HybridHeteromeric Protein ComplexDNA InteractionFunctional GenomicsProtein-DNA InteractionsCo-transformationTE Lithium AcetateYPDA MediumCompetent CellsNormalization Control

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Tripathi, P., Pruneda-Paz, J. L., Kay, S. A. A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies. J. Vis. Exp. (125), e56080, doi:10.3791/56080 (2017).

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