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A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies
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Biochemie
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JoVE Journal Biochemie
A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies

A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies

11,122 Views

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10:47 min

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July 24, 2017

DOI:

10.3791/56080-v

DOI:
10.3791/56080-v

•

10:47 min
July 24, 2017

•

6 Views
, ,
1Department of Neurology,University of Southern California, 2Section of Cell and Developmental Biology,University of California San Diego

Summary

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The modified yeast one-hybrid assay described here is an extension of the classical yeast one-hybrid (Y1H) assay to study and validate the heteromeric protein complex-DNA interaction in a heterologous system for any functional genomics study.

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Cite this Article

Tripathi, P., Pruneda-Paz, J. L., Kay, S. A. A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies. J. Vis. Exp. (125), e56080, doi:10.3791/56080 (2017).

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