Journal
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发育生物学
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High-resolution Episcopic Microscopy (HREM) – Simple and Robust Protocols for Processing and Visualizing Organic Materials
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
High-resolution Episcopic Microscopy (HREM) – Simple and Robust Protocols for Processing and Visualizing Organic Materials

High-resolution Episcopic Microscopy (HREM) – Simple and Robust Protocols for Processing and Visualizing Organic Materials

DOI:
10.3791/56071-v

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08:52 min

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July 07, 2017

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, , ,
1Division of Anatomy, Center for Anatomy and Cell Biology & MIC,Medical University of Vienna

Chapters

  • 00:05Title
  • 01:33Preparation of Embryonic Tissues
  • 02:58Embedding
  • 04:36High-resolution Episcopic Microscopy
  • 05:52Results: Visualization of Embryo and Adult Tissue Samples
  • 07:42Conclusion

Summary

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We provide simple and robust protocols for processing biopsy material of various species, embryos of biomedical model organisms and samples of other organic tissues in order to permit digital volume data generation with the high-resolution episcopic microscopy method.

Tags

High-resolution Episcopic Microscopy (HREM)Organic Materials3D AnalysisVisualizationBiomedical ResearchDehydrationInfiltrationEmbeddingVolume DataModel OrganismsWound Healing

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