需要订阅 JoVE 才能查看此. 登录或开始免费试用。
A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation
8,730 Views
•
10:04 min
•
July 06, 2017
DOI:
Summary
Automatically generated
We present a filtration-based protocol to isolate high-quality nuclei from cross-linked mouse skeletal muscle wherein we removed the need for ultracentrifugation, making it easily applicable. We show that chromatin prepared from the nuclei is suitable for chromatin immunoprecipitation and likely chromatin immunoprecipitation sequencing studies.
Read Article
Cite this Article
Nohara, K., Chen, Z., Yoo, S. A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation. J. Vis. Exp. (125), e56013, doi:10.3791/56013 (2017).
Copy