/

/

小鼠小脑颗粒神经元神经元死亡和变性的模型研究

JoVE 杂志
神经科学

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志神经科学

Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

Please note that all translations are automatically generated. Click here for the English version.

小鼠小脑颗粒神经元神经元死亡和变性的模型研究

7,912 Views

•

10:36 min

•

November 06, 2017

DOI:

10.3791/55871-v

DOI:

10.3791/55871-v

•

10:36 min

November 06, 2017

•

7 Views

Matthew Laaper^1,2, Takrima Haque¹, Ruth S. Slack³, Arezu Jahani-Asl^1,2,4

¹Lady Davis Institute for Medical Research,Jewish General Hospital, ²Integrated Program in Neuroscience,McGill University, ³Department of Cellular and Molecular Medicine,University of Ottawa, ⁴Department of Oncology, Faculty of Medicine,McGill University

Summary

本协议描述了一种简单的方法, 分离和培养的小鼠脑颗粒神经元 (CGNs) 从6-7 天的老幼崽, 有效的转导 CGNs 的损失和增益的功能研究, 并建模 NMDA 诱导神经元兴奋,低诱导的细胞死亡, DNA 损伤, 和氧化应激使用相同的培养模式。

Explore More Videos

Cerebellar Granule Neurons Neuronal Death Neurodegeneration Primary Cell Culture Excitotoxicity Oxidative Stress DNA Damage Developmental Events Neuronal Activity Morphogenesis Growth Factors Mouse Brain Dissection Cerebellum Isolation Meningeal Removal

Read Article