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生物化学
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Microcrystallography of Protein Crystals and In Cellulo Diffraction
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Microcrystallography of Protein Crystals and In Cellulo Diffraction

Microcrystallography of Protein Crystals and In Cellulo Diffraction

DOI:
10.3791/55793-v

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09:35 min

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July 21, 2017

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, ,
1Infection and Immunity Program, Monash Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology,Monash University

Chapters

  • 00:05Title
  • 01:12Identification of Crystal-containing Cells
  • 02:46Purification of Crystal-containing Cells
  • 05:00Sample Preparation for Data Collection
  • 06:47Data Collection
  • 07:42Results
  • 08:53Conclusion

Summary

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A protocol is presented for X-ray crystallography using protein microcrystals. Two examples analyzing in vivo-grown microcrystals after purification or in cellulo are compared.

Tags

MicrocrystallographyProtein CrystalsIn Cellulo DiffractionX-ray CrystallographyMicrocrystalsIn Vivo CrystalsSf9 CellsCypovirus Polyhedron ProteinFlow CytometryPropidium Iodide

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