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Chapters
- 00:00Title
- 00:37LEW.1W to LEW.1A Allograft Procedure
- 02:18Isolation of Allograft APCs (pDCs)
- 06:12Isolation of Responder Effector T Cells and Regulatory T Cells (CD4+ and CD8+CD45RClow)
- 10:30CFSE Labeling of Responder Cells to Measure their Proliferation
- 12:09FACS Staining and Analysis After 6 Days of Coculture
- 13:49In Vivo Assessment of the Cells Supressive Activity by Adoptive Cell Transfer in a Heart Grafted Recipient
- 16:33Representative Results
- 18:03Conclusion
