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A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
JoVE 杂志
生物工程
This content is Free Access.
JoVE 杂志 生物工程
A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

DOI:
10.3791/55351-v

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09:38 min

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February 14, 2017

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, , , , ,
1Transfusion Research Center,Belgian Red Cross-Flanders, 2Faculty of Medicine and Health Sciences,Ghent University, 3Blood Service,Belgian Red Cross-Flanders, 4Department of Public Health and Primary Care,KULeuven – University of Leuven

Chapters

  • 00:05Title
  • 01:01Microfluidics Setup
  • 03:56Blood Sample Collection and Preparation
  • 05:33Perfusion Assay
  • 07:06Results: Representative Platelet Adhesion and Coagulation analyses
  • 08:49Conclusion

Summary

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This paper describes an experimental model of hemostasis that simultaneously measures platelet function and coagulation. Platelet and fibrin fluorescence is measured in real-time, and platelet adhesion rate, coagulation rate, and onset of coagulation are determined. The model is used to determine platelet procoagulant properties under flow in concentrates for transfusion.

Tags

Microfluidic Flow ChamberPlatelet TransfusionHemostasisPlatelet DepositionFibrin FormationReal-time Video MicroscopyTransfusion MedicinePlatelet FunctionCoagulationContact PathwayExtrinsic PathwayCollagenBlocking BufferNanopump SoftwareHEPES Buffered SalineMicroscope Stage

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