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A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
JoVE Journal
Bioingegneria
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JoVE Journal Bioingegneria
A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

13,216 Views

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09:38 min

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February 14, 2017

DOI:

10.3791/55351-v

DOI:
10.3791/55351-v

•

09:38 min
February 14, 2017

•

6 Views
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1Transfusion Research Center,Belgian Red Cross-Flanders, 2Faculty of Medicine and Health Sciences,Ghent University, 3Blood Service,Belgian Red Cross-Flanders, 4Department of Public Health and Primary Care,KULeuven – University of Leuven

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This paper describes an experimental model of hemostasis that simultaneously measures platelet function and coagulation. Platelet and fibrin fluorescence is measured in real-time, and platelet adhesion rate, coagulation rate, and onset of coagulation are determined. The model is used to determine platelet procoagulant properties under flow in concentrates for transfusion.

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Six, K. R., Devloo, R., Van Aelst, B., Vandekerckhove, P., Feys, H. B., Compernolle, V. A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time. J. Vis. Exp. (120), e55351, doi:10.3791/55351 (2017).

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