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在荧光蛋白融合的一代<em>念珠菌</em>物种
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Generation of Fluorescent Protein Fusions in Candida Species
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在荧光蛋白融合的一代<em>念珠菌</em>物种

10,483 Views

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09:27 min

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March 04, 2017

DOI:

10.3791/55333-v

DOI:
10.3791/55333-v

•

09:27 min
March 04, 2017

•

6 Views
, ,
1Department of Pediatrics,University of Minnesota, 2Department of Molecular Microbiology and Biotechnology,Tel Aviv University

Summary

Automatically generated

PCR介导的基因修饰可用于产生在念珠菌属种类，这有利于可视化和酵母细胞和蛋白质的定量荧光蛋白融合体。在此，我们提出了在近平滑念珠菌构建荧光蛋白融合（左心室Eno1-FP）的战略。

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Cite this Article

Gonia, S., Berman, J., Gale, C. A. Generation of Fluorescent Protein Fusions in Candida Species. J. Vis. Exp. (121), e55333, doi:10.3791/55333 (2017).

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