Journal
/
神经科学
/
Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry

DOI:
10.3791/55254-v

•

08:36 min

•

March 23, 2017

•

, , , ,
1Department of Anatomy and Cell Biology,The University of Western Ontario, 2Department of Chemistry and Biochemistry,The University of Western Ontario

Chapters

  • 00:05Title
  • 00:42Tissue Preparation and Sectioning
  • 02:08Sublimator Apparatus Set-up
  • 04:57Sublimation
  • 06:57Results: Matrix-assisted Laser Desorption/Ionization (MALDI) Imaging Mass Spectrometry (IMS) of Gangliosides in the Rat Brain
  • 07:31Conclusion

Summary

自动翻译

自动翻译

A protocol for the sublimation of DAN matrix onto rat brain tissue for the detection of gangliosides using MALDI Imaging Mass Spectrometry is presented.

Tags

GangliosidesMALDI Imaging Mass SpectrometrySublimationDAN MatrixCryostat SectioningLipid DetectionNeurobiology

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/55254?article_type=v

Visualization of Proprioceptors in Drosophila Larvae and Pupae
check_url/cn/55254?article_type=v

Stereotaxic Surgery for Excitotoxic Lesion of Specific Brain Areas in the Adult Rat
check_url/cn/55254?article_type=v

Fiber-optic Implantation for Chronic Optogenetic Stimulation of Brain Tissue
check_url/cn/55254?article_type=v

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
check_url/cn/55254?article_type=v

A Dual Tracer PET-MRI Protocol for the Quantitative Measure of Regional Brain Energy Substrates Uptake in the Rat
check_url/cn/55254?article_type=v

Non-invasive Parenchymal, Vascular and Metabolic High-frequency Ultrasound and Photoacoustic Rat Deep Brain Imaging
check_url/cn/55254?article_type=v

Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry
check_url/cn/55254?article_type=v

High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue
check_url/cn/55254?article_type=v

Labeling and Imaging of Amyloid Plaques in Brain Tissue Using the Natural Polyphenol Curcumin
check_url/cn/55254?article_type=v

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

Read Article