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Measuring Neuromuscular Junction Functionality
Chapters
- 00:00Title
- 00:14Introduction
- 00:40Ex Vivo Experimental System
- 02:57Dissection of Soleus Muscle and Nerve
- 04:04Mounting the Soleus in the Apparatus
- 05:27Testing Protocol and Data Collection
- 05:47Twitch
- 06:09Force Frequency Measurement
- 06:27Fatigue Paradigm
- 07:04Neurotransmission Functionality Specific Parameters
- 07:56Results
- 09:40Conclusion
A functional assessment of the neuromuscular junction (NMJ) can provide essential information on the communication between muscle and nerve. Here we describe a protocol to comprehensively evaluate both the NMJ and muscle functionality using two different muscle-nerve preparations, i.e. soleus-sciatic and diaphragm-phrenic.
Tags
Neuromuscular Junction FunctionalityEx Vivo Experimental ApproachMuscle Nerve PreparationMembrane StimulationNerve StimulationContractile ResponsesSoleus Sciatic Nerve PreparationForce And Length ControllerPlatinum ElectrodesGlass Suction ElectrodeTesting ProtocolMuscle FunctionalityPathological ConditionsKrebs-Ringer SolutionGas MixtureActuator TransducerPulse StimulatorsMuscle MembraneNerve StimulationSacrificingCervical DislocationGastrocnemius MuscleSoleus MuscleProximal Tendon
