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和无血清收集无饲养小鼠胚胎干细胞条件培养基的无细胞方法
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09:32 min
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January 08, 2017
DOI:
1Department of Biochemistry and Molecular Biology and Smart-aging Convergence Research Center, College of Medicine,Yeungnam University, 2Physiology and Experimental Medicine Program,The Hospital for Sick Children Research Institute, 3Department of Laboratory Medicine and Pathobiology,University of Toronto
Summary
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这个协议提供了小鼠胚胎干细胞的血清衍生的集合（MESC）-conditioned培养基（MESC-CM）的方法（胎牛血清，FBS） - 和馈线（小鼠胚胎成纤维细胞，MEF中）用于小区-free条件 - 免费的做法。它可以是适用于老化和老化有关的疾病的治疗。
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Cite this Article
Bae, Y., Sung, H., Kim, J. Collection of Serum- and Feeder-free Mouse Embryonic Stem Cell-conditioned Medium for a Cell-free Approach. J. Vis. Exp. (119), e55035, doi:10.3791/55035 (2017).
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