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비계 리포좀을 사용하여 근위 지질 단백질 - 단백질 상호 작용을 재구성<em> 체외</em
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生物化学
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Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
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비계 리포좀을 사용하여 근위 지질 단백질 - 단백질 상호 작용을 재구성<em> 체외</em

8,583 Views

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08:53 min

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January 11, 2017

DOI:

10.3791/54971-v

DOI:
10.3791/54971-v

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08:53 min
January 11, 2017

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6 Views
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1Department of Pharmacology, Center for Mitochondrial Diseases, The Cleveland Center for Membrane and Structural Biology,Case Western Reserve University School of Medicine

Summary

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This paper describes a method for assessing the interactions and assemblies of integral membrane proteins in vitro with various partner factors in a lipid-proximal environment.

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Cite this Article

Clinton, R. W., Mears, J. A. Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro. J. Vis. Exp. (119), e54971, doi:10.3791/54971 (2017).

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