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发育生物学

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诱导多能干细胞的快速神经元分化有关微电极阵列测量网络活动

JoVE 杂志
发育生物学

This content is Free Access.

JoVE 杂志发育生物学

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

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诱导多能干细胞的快速神经元分化有关微电极阵列测量网络活动

26,085 Views

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09:20 min

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January 08, 2017

DOI:

10.3791/54900-v

DOI:

10.3791/54900-v

•

09:20 min

January 08, 2017

•

24 Views

Monica Frega*^1,2, Sebastianus H. C. van Gestel*³, Katrin Linda^2,3, Jori van der Raadt³, Jason Keller^1,2, Jon-Ruben Van Rhijn^1,2, Dirk Schubert^1,2, Cornelis A. Albers^2,3,4, Nael Nadif Kasri^1,2,3

¹Department of Cognitive Neurosciences,Radboudumc, ²Donders Institute for Brain, Cognition and Behaviour,Radboud University, ³Department of Human Genetics,Radboudumc, ⁴Department of Molecular Developmental Biology,Radboud University

Summary

我们修改和实施描述人类诱导多能干细胞（iPS细胞）的快速，可重复，高效分化成兴奋皮层神经元¹²先前公布的协议。具体地讲，我们的修改允许对神经元细胞密度和使用的控制上的微电极阵列以测量在网络级电生理特性。

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