Measurement of Differentially Methylated <em>INS</em> DNA Species in Human Serum Samples as a Biomarker of Islet &#946; Cell Death

Sarah A Tersey; Jennifer B Nelson; Marisa M Fisher; Raghavendra G Mirmira

doi:10.3791/54838

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遗传学

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Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death

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Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death

Measurement of Differentially Methylated INS DNA Species in Human Serum Samples as a Biomarker of Islet β Cell Death

DOI:

10.3791/54838-v

•

10:34 min

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December 21, 2016

•

Sarah A Tersey, Jennifer B Nelson, Marisa M Fisher, Raghavendra G Mirmira^3,4

¹Department of Pediatrics, IU Center for Diabetes and Metabolic Disease,Indiana University School of Medicine, ²Department of Pediartics, Omaha Children’s Hospital and Medical Center,University of Nebraska Medical Center, ³Departments of Biochemistry and Molecular Biology, Medicine, and Cellular and Integrative Physiology, IU Center for Diabetes and Metabolic Disease,Indiana University School of Medicine, ⁴Indiana Biosciences Research Institute

Chapters

00:05Title
00:42Serum DNA Extraction
03:01Bisulfite Conversion
04:59Multiplex Digital PCR
08:00Results: Differentially Methylated INS DNA Measurements
09:25Conclusion

Summary

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Islet β cell death precedes development of type 1 diabetes, and detecting this process may allow for early therapeutic intervention. Here, we provide a detailed description of how to measure differentially methylated INS DNA species in human serum as a biomarker of β cell death.

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