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发育生物学
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Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies
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发育生物学
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JoVE 杂志 发育生物学
Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies

Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies

DOI:
10.3791/54764-v

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04:42 min

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December 29, 2016

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, , , ,
1Biotechnology and Genetic Engineering Department,Jahangirnagar University, 2JT Biohistory Research Hall, 3Division of Cell Regeneration and Transplantation, School of Medicine,Nihon University, 4Graduate School of Life and Medical Sciences,Doshisha University, 5Institute of Genetics and Animal Breeding of the Polish Academy of Sciences

Chapters

  • 00:05Title
  • 00:39Apple Plate Preparation
  • 01:24Embryo Preparation
  • 02:30Live Imaging
  • 03:43Results
  • 04:13Conclusion

Summary

自动翻译

自动翻译

Herein we describe a procedure to capture live images of Drosophila gastrulation. This has enabled us to better understand the apical constriction involved in early development and further analyze mechanisms governing cellular movements during tissue structure modification.

Tags

Keywords: Cell ShapeCell MovementDrosophila GastrulationDE-cadherinReporter Transgenic FliesCellular RearrangementApical ConstrictionEmbryonic DevelopmentImagingMicroscopySample PreparationEmbryo CollectionDechorionationOrientationMounting

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