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Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies
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Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies
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04:42 min
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December 29, 2016
DOI:
1Biotechnology and Genetic Engineering Department,Jahangirnagar University, 2JT Biohistory Research Hall, 3Division of Cell Regeneration and Transplantation, School of Medicine,Nihon University, 4Graduate School of Life and Medical Sciences,Doshisha University, 5Institute of Genetics and Animal Breeding of the Polish Academy of Sciences
Summary
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Herein we describe a procedure to capture live images of Drosophila gastrulation. This has enabled us to better understand the apical constriction involved in early development and further analyze mechanisms governing cellular movements during tissue structure modification.
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Karim, M. R., Haruta, T., Matsumoto, T., Oda, H., Taniguchi, H. Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies. J. Vis. Exp. (118), e54764, doi:10.3791/54764 (2016).
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