Journal
/
免疫与感染
/
Фагосоме Миграция и скорость, измеренная в живых первичных макрофагах человека, инфицированных ВИЧ-1
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1
Please note that all translations are automatically generated. Click here for the English version.

Фагосоме Миграция и скорость, измеренная в живых первичных макрофагах человека, инфицированных ВИЧ-1

8,066 Views

•

07:32 min

•

September 05, 2016

DOI:

10.3791/54568-v

DOI:
10.3791/54568-v

•

07:32 min
September 05, 2016

•

2 Views
, , ,
1Inserm U1016, Institut Cochin, CNRS UMR 8104, Université Paris Descartes, Sorbonne Paris Cité

Summary

Automatically generated

We describe a method to measure the velocity of phagosomes moving towards the cell center in living cells infected with or without the human immunodeficiency virus (HIV) type 1, using spinning disk confocal fluorescence microscopy to identify fluorescent infected cells and bright field microscopy to detect phagosomes.

Explore More Videos

Phagosome MigrationPhagosome VelocityHIV-1 Infected MacrophagesLive Cell ImagingConfocal MicroscopyPhagocytosis AssaySheep-bred Blood CellsGFP-positiveTime-lapse VideoVideo Editing SoftwareManual Tracking Method

Read Article

Cite this Article

Lê-Bury, G., Deschamps, C., Dumas, A., Niedergang, F. Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1. J. Vis. Exp. (115), e54568, doi:10.3791/54568 (2016).

Download .ris file

Copy

Share Video

.

Copy