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Migrazione phagosome e velocità misurata in Live primarie macrofagi umani infettati con HIV-1
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JoVE 杂志 免疫与感染
Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1
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Migrazione phagosome e velocità misurata in Live primarie macrofagi umani infettati con HIV-1

8,066 Views

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07:32 min

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September 05, 2016

DOI:

10.3791/54568-v

DOI:
10.3791/54568-v

•

07:32 min
September 05, 2016

•

2 Views
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1Inserm U1016, Institut Cochin, CNRS UMR 8104, Université Paris Descartes, Sorbonne Paris Cité

Summary

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We describe a method to measure the velocity of phagosomes moving towards the cell center in living cells infected with or without the human immunodeficiency virus (HIV) type 1, using spinning disk confocal fluorescence microscopy to identify fluorescent infected cells and bright field microscopy to detect phagosomes.

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Cite this Article

Lê-Bury, G., Deschamps, C., Dumas, A., Niedergang, F. Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1. J. Vis. Exp. (115), e54568, doi:10.3791/54568 (2016).

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