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Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
Chapters
- 00:05Title
- 00:47Peptide Serial Dilution
- 03:24Cell Preparation
- 04:24Homogeneous Time Resolved Fluorescence cAMP Detection Assay
- 05:41Results: Potency Determination Assay Using Acoustic Transfer to Serially Dilute Peptide Agonists
- 06:59Conclusion
Peptide adsorption to plasticware during traditional tip-based serial dilutions can significantly impact potency determination and confound the understanding of structure-activity relationships used for lead identification and lead optimization phases of drug discovery. Here methods for automated acoustic non-contact serial dilution of peptide samples are described.
