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Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment
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JoVE 杂志 发育生物学
Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment

Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment

DOI:
10.3791/54492-v

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11:19 min

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December 16, 2016

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, , ,
1Laboratory of Genetics,University of Wisconsin, 2Department of Neurobiology,University of Massachusetts Medical School, 3Interdisciplinary Biomedical Graduate Program,University of Pittsburgh School of Medicine

Chapters

  • 00:05Title
  • 00:47Selecting Females for Egg Collection via Interrupted Mating
  • 01:31Preparation of Sperm Solution
  • 02:50UV Treatment of Sperm Solution
  • 03:37Manual Extrusion of Mature Eggs
  • 04:56In Vitro Fertilization
  • 05:51Heat Shock Treatment
  • 07:13Selection of Embryos with a One-cycle Cytokinesis Stall
  • 08:48Results: Heat Shock 2 Treatment of Zebrafish Embryos
  • 09:57Conclusion

Summary

自动翻译

自动翻译

A modified protocol for ploidy manipulation uses a heat shock to induce a one-cycle stall in cytokinesis in the early embryo. This protocol is demonstrated in the zebrafish but may be applicable to other species.

Tags

Ploidy ManipulationZebrafish EmbryosHeat ShockDiploidization遗传学Inbred LinesGenetic ManipulationsMatingEgg ExtrusionSperm SolutionUV TreatmentDissectionTestesHank SolutionPipettingUV Lamp

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