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La fusión absoluta y relativa de datos de PCR cuantitativa para cuantificar STAT3 variante de empalme transcripciones
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts
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La fusión absoluta y relativa de datos de PCR cuantitativa para cuantificar STAT3 variante de empalme transcripciones

14,483 Views

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11:19 min

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October 09, 2016

DOI:

10.3791/54473-v

DOI:
10.3791/54473-v

•

11:19 min
October 09, 2016

•

3 Views
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1Department of Biomolecular Chemistry,University of Wisconsin-Madison, 2Department of Medicine,University of Wisconsin-Madison

Tags

Quantitative PCRSTAT3 Splice VariantsEosinophilsAbsolute QuantificationRelative QuantificationPlasmid StandardsCytokine StimulationCDNASequencingPlasmid ConcentrationCopy NumberDilution SeriesNon-target ControlsTarget MixPrimer Pairs

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Turton, K. B., Esnault, S., Delain, L. P., Mosher, D. F. Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts. J. Vis. Exp. (116), e54473, doi:10.3791/54473 (2016).

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