/

/

विलय निरपेक्ष और सापेक्ष मात्रात्मक पीसीआर डेटा Stat3 ब्याह संस्करण देखिए यों

JoVE 杂志
遗传学

This content is Free Access.

JoVE 杂志遗传学

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Please note that all translations are automatically generated. Click here for the English version.

विलय निरपेक्ष और सापेक्ष मात्रात्मक पीसीआर डेटा Stat3 ब्याह संस्करण देखिए यों

14,483 Views

•

11:19 min

•

October 09, 2016

DOI:

10.3791/54473-v

DOI:

10.3791/54473-v

•

11:19 min

October 09, 2016

•

3 Views

Keren B. Turton, Stephane Esnault, Larissa P. Delain, Deane F. Mosher²

¹Department of Biomolecular Chemistry,University of Wisconsin-Madison, ²Department of Medicine,University of Wisconsin-Madison

Tags

Quantitative PCR STAT3 Splice Variants Eosinophils Absolute Quantification Relative Quantification Plasmid Standards Cytokine Stimulation CDNA Sequencing Plasmid Concentration Copy Number Dilution Series Non-target Controls Target Mix Primer Pairs

Read Article