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合并绝对和相对定量PCR数据来量化STAT3剪接变体成绩单

JoVE 杂志
遗传学

This content is Free Access.

JoVE 杂志遗传学

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

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合并绝对和相对定量PCR数据来量化STAT3剪接变体成绩单

14,483 Views

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11:19 min

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October 09, 2016

DOI:

10.3791/54473-v

DOI:

10.3791/54473-v

•

11:19 min

October 09, 2016

•

3 Views

Keren B. Turton, Stephane Esnault, Larissa P. Delain, Deane F. Mosher²

¹Department of Biomolecular Chemistry,University of Wisconsin-Madison, ²Department of Medicine,University of Wisconsin-Madison

Tags

Quantitative PCR STAT3 Splice Variants Eosinophils Absolute Quantification Relative Quantification Plasmid Standards Cytokine Stimulation CDNA Sequencing Plasmid Concentration Copy Number Dilution Series Non-target Controls Target Mix Primer Pairs

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