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在微孔聚集尺寸优化人类多能干细胞的基于悬浮心脏分化
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells
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在微孔聚集尺寸优化人类多能干细胞的基于悬浮心脏分化

6,624 Views

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06:28 min

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September 25, 2016

DOI:

10.3791/54308-v

DOI:
10.3791/54308-v

•

06:28 min
September 25, 2016

•

1 Views
, ,
1Institute of Biomaterials and Biomedical Engineering,University of Toronto, 2Department of Comparative Biology and Experimental Medicine,University of Calgary

Summary

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常规的方法以引发人多能的悬浮聚合基于心脏分化干细胞（hPSCs）的困扰与培养异质相对于聚集体的尺寸和形状。在这里，我们描述了采用微孔产生大小控制HPSC心脏推进的条件下培养聚集心脏分化一个可靠的方法。

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Aggregate Size OptimizationMicrowellsSuspension-based Cardiac DifferentiationHuman Pluripotent Stem CellsCentrifugationCell SuspensionMicrowell PlateHypoxic IncubationAggregate HarvestingInduction Medium

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Cite this Article

Bauwens, C. L., Toms, D., Ungrin, M. Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells. J. Vis. Exp. (115), e54308, doi:10.3791/54308 (2016).

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