Waiting
登录处理中...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Large-Scale Production of Cardiomyocytes from…
JoVE Journal
Developmental Biology

需要订阅 JoVE 才能查看此 内容. 登录或开始免费试用。

JoVE Journal Developmental Biology
Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol
 

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol

Article DOI: 10.3791/54276-v • 12:21 min • July 25th, 2016
July 25th, 2016 •
*1,2,3,8, *1,8, 1, 6,7, 1, 6,7, 2,3,4, *2,3, *1,5
1Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, 2Developmental and Stem Cell Biology Division, Victor Chang Cardiac Research Institute, 3St. Vincent´s Clinical School, Faculty of Medicine, University of New South Wales, 4School of Biotechnology and Biomolecular Sciences, University of New South Wales, 5Department of Developmental Biology, University of Science and Culture, 6Heart Centre for Children, The Children´s Hospital at Westmead, 7Sydney Medical School, University of Sydney, 8Department of Developmental Biology, University of Science and Culture, Tehran, Iran
* These authors contributed equally

章节

总结概括
Automatic Translation

Here, we present a robust, fast and scalable cardiomyocyte differentiation protocol for human pluripotent stem cells (hPSCs). Cardiomyocytes derived using this large-scale method can provide sufficient cell numbers for their effective use in human cardiovascular disease modeling, high-throughput drug screening, and potentially clinical applications.

Tags

Keyword Extraction: Cardiomyocytes Human Pluripotent Stem Cells Differentiation Protocol High Efficiency Reproducibility Cardiac Disease Modeling Human Induced Pluripotent Stem Cells Cost-effective Human Embryonic Stem Cells Single Cell Passaging Spheroids PBS Trypsin EDTA
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter