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Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System
 

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

Article DOI: 10.3791/54273-v • 11:47 min • August 1st, 2016
August 1st, 2016 •
1, 2, 2, 1, 3, 2, 1
1Department of Experimental Physics, Saarland University, 2Institute of Chemistry, Technische Universität Berlin, 3School of Physics and Astronomy, University of Minnesota

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An easy-to-use, cell-free expression protocol for the residue-specific incorporation of noncanonical amino acid analogs into proteins, including downstream analysis, is presented for medical, pharmaceutic, structural and functional studies.

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Keywords: Noncanonical Amino Acids Cell-free Expression Protein Structure Residue-specific Incorporation Amino Acid Master Mix CAA NcAA Cell-free Transcription-translation System
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