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Chapters
- 00:05Title
- 00:54Reverse siRNA Transfection
- 03:19Infection and Fixation
- 05:55Staining and Imaging
- 08:14Automated Image Analysis
- 12:21Results: Automated High-throughput Quantification of Brucella Infection in HeLa Cells
- 14:06Conclusion
Two assays for microscopy-based high-throughput screening of host factors involved in Brucella infection are described. The entry assay detects host factors required for Brucella entry and the endpoint assay those required for intracellular replication. While applicable for alternative approaches, siRNA screening in HeLa cells is used to illustrate the protocols.
