需要订阅 JoVE 才能查看此. 登录或开始免费试用。
在参与宿主因素的高通量筛选基于显微镜的检测方法<em>布鲁氏菌</em> Hela细胞感染
8,137 Views
•
15:29 min
•
August 05, 2016
DOI:
Summary
Automatically generated
Two assays for microscopy-based high-throughput screening of host factors involved in Brucella infection are described. The entry assay detects host factors required for Brucella entry and the endpoint assay those required for intracellular replication. While applicable for alternative approaches, siRNA screening in HeLa cells is used to illustrate the protocols.
Read Article
Cite this Article
Casanova, A., Low, S. H., Emmenlauer, M., Conde-Alvarez, R., Salcedo, S. P., Gorvel, J., Dehio, C. Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells. J. Vis. Exp. (114), e54263, doi:10.3791/54263 (2016).
Copy