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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
JoVE 杂志
医学
This content is Free Access.
JoVE 杂志 医学
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

DOI:
10.3791/53836-v

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13:24 min

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April 11, 2016

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, , , ,
1Asuragen, Inc.

Chapters

  • 00:05Title
  • 01:06DNA Functional Quantification and Quality Control
  • 02:55Library Preparation
  • 05:22Library Purification and Size Selection
  • 06:50Library Quantification
  • 08:21Library Normalization and Sample Pooling
  • 09:23Sequencing
  • 11:09Results: High-depth, Multiplexed Sequencing and Sensitive Analysis of Challenging Cancer Specimens by Targeted NGS
  • 13:01Conclusion

Summary

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An integrated system for targeted next-generation sequencing of oncology specimens is described. This cross-platform system is optimized for low-quality and low-quantity tumor biopsies, accommodates low DNA inputs, includes well-characterized multi-variant controls, and features a novel variant caller that is informed by quantitative pre-analytical quality control measures.

Tags

Keywords: Targeted Next-generation SequencingLow-quality Tumor BiopsiesLow-quantity Tumor BiopsiesDNA Variant AnalysisQuantificationQualificationReal-time PCRDNA Template CopiesLibrary QuantificationSample PoolingWet And Dry Bench ProcessesBioinformatics

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