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Teknik Target Mikroinjektion til udviklingslandene<em> Xenopus</em> Nyre
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Technique to Target Microinjection to the Developing Xenopus Kidney
DOI:

11:29 min

May 03, 2016

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Chapters

  • 00:05Title
  • 00:44Preparation of Embryos
  • 03:32Preparation of Injection Solutions and Microinjection of Embryos
  • 08:22Fixation and Immunostaining of Embryos
  • 09:42Results: Examples of Embryos Targeted at the 8-cell Stage
  • 10:56Conclusion

Summary

自动翻译

Here, we present a protocol to use fate maps and lineage tracers to target injections into individual blastomeres that give rise to the kidney of Xenopus laevis embryos.

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