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大鼠少突胶质祖文化的制备和使用双红外荧光扫描Oligodendrogenesis的量化
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发育生物学
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JoVE 杂志 发育生物学
Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning
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大鼠少突胶质祖文化的制备和使用双红外荧光扫描Oligodendrogenesis的量化

11,543 Views

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11:11 min

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February 17, 2016

DOI:

10.3791/53764-v

DOI:
10.3791/53764-v

•

11:11 min
February 17, 2016

•

8 Views
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1Neurology,Johns Hopkins University, School of Medicine

Summary

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Oligodendrocytes are the myelinating cells of the central nervous system. This protocol describes a method for the isolation and culture of their precursors, oligodendrocyte progenitor cells, from rat cortices, as well as a fast and reliable quantitative method to evaluate oligodendrogenesis in vitro in response to experimental factors.

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Cite this Article

Schott, J. T., Kirby, L. A., Calabresi, P. A., Baxi, E. G. Preparation of Rat Oligodendrocyte Progenitor Cultures and Quantification of Oligodendrogenesis Using Dual-infrared Fluorescence Scanning. J. Vis. Exp. (108), e53764, doi:10.3791/53764 (2016).

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