Préparation de cultures Rat oligodendrocytes progénitrices et la quantification des oligodendrogenèse par analyse de fluorescence à double infrarouge

Oligodendrocytes are the myelinating cells of the central nervous system. This protocol describes a method for the isolation and culture of their precursors, oligodendrocyte progenitor cells, from rat cortices, as well as a fast and reliable quantitative method to evaluate oligodendrogenesis in vitro in response to experimental factors.