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Eine einfache Alternative zur stereotaktische Injektion für Hirnspezifische Knockdown von miRNA
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神经科学
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JoVE 杂志 神经科学
A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
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Eine einfache Alternative zur stereotaktische Injektion für Hirnspezifische Knockdown von miRNA

12,539 Views

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06:53 min

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December 26, 2015

DOI:

10.3791/53307-v

DOI:
10.3791/53307-v

•

06:53 min
December 26, 2015

•

6 Views
, , , , , ,
1CSIR-Institute of Genomics and Integrative Biology, New Delhi, India, 2Academy of Scientific and Innovative Research (AcSIR), New Delhi, India

Summary

Automatically generated

MicroRNAs play crucial roles in the brain and are potential targets for modeling neuro-degeneration. However, perturbing miRNA levels is challenging due to the short length of miRNA and inaccessibility of the brain tissue. This video presents a method for antagomir design and brain specific delivery using a neuropeptide in mice.

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MiRNAMicroRNAAntagomirLNARVGBrain DeliveryStereotactic InjectionNeurodegenerative DiseaseNeuronal Cell DeathGene ExpressionNeurodevelopmentNeuronal Function

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Cite this Article

Suryawanshi, H., Sarangdhar, M. A., Vij, M., Roshan, R., Singh, V. P., Ganguli, M., Pillai, B. A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA. J. Vis. Exp. (106), e53307, doi:10.3791/53307 (2015).

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