Journal
/
神经科学
/
一个简单的替代，以立体定向注射miRNA的脑特异性敲除
JoVE 杂志
神经科学
Author Produced
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
Please note that all translations are automatically generated. Click here for the English version.

一个简单的替代，以立体定向注射miRNA的脑特异性敲除

12,538 Views

•

06:53 min

•

December 26, 2015

DOI:

10.3791/53307-v

DOI:
10.3791/53307-v

•

06:53 min
December 26, 2015

•

6 Views
, , , , , ,
1CSIR-Institute of Genomics and Integrative Biology, New Delhi, India, 2Academy of Scientific and Innovative Research (AcSIR), New Delhi, India

Summary

Automatically generated

MicroRNAs play crucial roles in the brain and are potential targets for modeling neuro-degeneration. However, perturbing miRNA levels is challenging due to the short length of miRNA and inaccessibility of the brain tissue. This video presents a method for antagomir design and brain specific delivery using a neuropeptide in mice.

Explore More Videos

MiRNAMicroRNAAntagomirLNARVGBrain DeliveryStereotactic InjectionNeurodegenerative DiseaseNeuronal Cell DeathGene ExpressionNeurodevelopmentNeuronal Function

Read Article

Cite this Article

Suryawanshi, H., Sarangdhar, M. A., Vij, M., Roshan, R., Singh, V. P., Ganguli, M., Pillai, B. A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA. J. Vis. Exp. (106), e53307, doi:10.3791/53307 (2015).

Download .ris file

Copy

Share Video

.

Copy