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An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics
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JoVE 杂志 免疫与感染
An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics

An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics

DOI:
10.3791/53288-v

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09:25 min

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December 24, 2015

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,
1Department of Medicine,Harvard Medical School – BIDMC

Chapters

  • 00:05Title
  • 01:06Endothelia and Lymphocyte Preparation
  • 02:55Live-cell Imaging Set Up
  • 05:37Th1 Effector/Memory-endothelial Cell Immunological Synapse (IS) Initiation
  • 06:56Results: Representative Th1:Endothelial APC ISs
  • 08:51Conclusion

Summary

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Adaptive immunity is controlled by dynamic 'immunological synapses' formed between T cells and antigen presenting cells. This protocol describes methods for investigating endothelial cells both as understudied physiologic APCs and as a novel type of 'planar cellular APC model'.

Tags

Endothelial CellsImmunological SynapseT CellsAntigen Presenting Cells (APCs)MHC/Ag-TCRF-actinAdhesion MoleculesSignaling MoleculesPlanar Cell ModelImagingAdaptive ImmunitySemi-professional APCsFluorescent Protein Reporters

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