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Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome
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JoVE 杂志 神经科学
Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

DOI:
10.3791/53165-v

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08:49 min

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September 23, 2015

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, , ,
1Ross University School of Veterinary Medicine, 2Charles E. Schmidt College of Medicine,Florida Atlantic University

Chapters

  • 00:05Title
  • 01:32Dehydration and Pretreatment
  • 03:32Hybridization and Amplification
  • 05:35Signal Detection
  • 06:20Image Particle Analysis
  • 07:31Results: A Light Microscopic View of Hybridization Signals and the Effect on Hypothalamic htr2a Expression in Rats Treated with MDMA
  • 08:16Conclusion

Summary

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This protocol describes a rapid and simplified in situ hybridization method ideal forparaformaldehyde-prefixed brain, thus reducing the need for prolonged complex steps while using fresh frozen tissues. The method is validated using the identification of the serotonin 5-HT2A receptor gene htr2a in rats.

Tags

Keywords: In Situ HybridizationOligonucleotide ProbesParaformaldehyde-fixed BrainSerotonin5-HT2A ReceptorMDMASerotonin Syndrome

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