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Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
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Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
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Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface

DOI:
10.3791/52866-v

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07:51 min

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May 21, 2015

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, , , ,
1Department of Obstetrics & Gynecology,Wayne State University School of Medicine, 2School of Paediatrics and Reproductive Health, Research Centre for Reproductive Health, the Robinson Research Institute,The University of Adelaide, 3Department of Immunology & Microbiology,Wayne State University School of Medicine, 4Perinatology Research Branch,NICHD/NIH/DHHS

Chapters

  • 00:05Title
  • 01:23Tissue Collection
  • 04:00Uterine, Decidual, and Placental Tissue Dissociation
  • 05:58Results: Leukocyte Populations in the Maternal-fetal Interface
  • 07:01Conclusion

Summary

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Described herein is a protocol to isolate and analyze the infiltrating leukocytes of tissues at the maternal-fetal interface (uterus, decidua, and placenta) of mice. This protocol maintains the integrity of most cell surface markers and yields enough viable cells for downstream applications including flow cytometry analysis.

Tags

Leukocyte IsolationMaternal-fetal InterfacePlacentaDeciduaUterusImmune TolerancePregnancyImmune CellsNeutrophilsMacrophagesDendritic CellsT CellsB CellsNK CellsNKT CellsEnzymatic Digestion

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