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Chapters
- 00:05Title
- 01:28Isolation of Cancer Stem Cells from Pancreatic Ductal Adenocarcinoma
- 02:58Sphere Formation Assay and Metformin Treatment
- 04:21Metabolism Analysis: ROS Production and Oxygen Consumption
- 06:09Results: The Effects of Metformin on Pancreatic CSC Function
- 06:59Conclusion
Pancreatic cancer stem cells (CSCs) can be expanded in vitro using the anchorage-independent sphere culture technique, which represents a powerful tool to study CSC biology and can serve as the first step to develop novel CSC-targeting therapies. Here the methodology for expanding, analyzing and targeting of pancreatic CSCs is provided.
