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Изучение рака поджелудочной железы стволовых клеток Характеристики для разработки новых стратегий лечения
JoVE 杂志
医学
This content is Free Access.
JoVE 杂志 医学
Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
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Изучение рака поджелудочной железы стволовых клеток Характеристики для разработки новых стратегий лечения

19,169 Views

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07:29 min

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June 20, 2015

DOI:

10.3791/52801-v

DOI:
10.3791/52801-v

•

07:29 min
June 20, 2015

•

2 Views
, , , ,
1Stem Cells & Cancer Group, Molecular Pathology Program,Spanish National Cancer Research Center, 2Institute for Research in Biomedicine (IRB Barcelona), 3Center for Stem Cells in Cancer & Ageing, Barts Cancer Institute,Queen Mary University of London

Summary

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Pancreatic cancer stem cells (CSCs) can be expanded in vitro using the anchorage-independent sphere culture technique, which represents a powerful tool to study CSC biology and can serve as the first step to develop novel CSC-targeting therapies. Here the methodology for expanding, analyzing and targeting of pancreatic CSCs is provided.

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Cite this Article

Lonardo, E., Cioffi, M., Sancho, P., Crusz, S., Heeschen, C. Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies. J. Vis. Exp. (100), e52801, doi:10.3791/52801 (2015).

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