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Påvisning av Axonally Lokalisert mRNAs i hjernedelene med høy oppløsning<em> In Situ</em> Hybridisering
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JoVE 杂志 神经科学
Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

Påvisning av Axonally Lokalisert mRNAs i hjernedelene med høy oppløsning<em> In Situ</em> Hybridisering

DOI:
10.3791/52799-v

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11:24 min

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June 17, 2015

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, , ,
1College of Physicians and Surgeons,Columbia University

Chapters

  • 00:05Title
  • 01:26Heat-Induced Antigen/RNA Unmasking
  • 02:21Probe Hybridization
  • 04:01Immunofluorescence Staining
  • 05:42Diaminobenzidine (DAB)-based Chromogenic ISH Assay Followed by Luxol Fast Blue and Cresyl Violet Stain Using Heat-induced and Protease-induced RNA Unmasking
  • 08:54Results: Aft4 Localization to Cholinergic Axons in the Mouse Dentate Gyrus and Myelinated Axons in the Adult Hippocampal Formation
  • 10:53Conclusion

Summary

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自动翻译

RNA in situ hybridization (ISH) enables the visualization of RNAs in cells and tissues. Here we show how combination of RNAscope ISH with immunohistochemistry or histological dyes can be successfully used to detect mRNAs localized to axons in sections of mouse and human brains.

Tags

Keywords: Axonally Localized MRNAsIn Situ HybridizationRNAscopeAxonal CounterstainFluorescence ImmunohistochemistryHistological DyesAtf4 MRNATranscriptome AnalysisVertebrate AxonsAxon PathfindingAxon BranchingNeurodegenerationSingle-molecule DetectionSubcellular LocalizationMature Mouse BrainHuman Brain

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