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Detectie van Axonally Gelokaliseerde mRNA in Brain secties met behulp van High-Resolution<em> In Situ</em> Hybridisatie
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JoVE 杂志 神经科学
Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
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Detectie van Axonally Gelokaliseerde mRNA in Brain secties met behulp van High-Resolution<em> In Situ</em> Hybridisatie

11,590 Views

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11:24 min

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June 17, 2015

DOI:

10.3791/52799-v

DOI:
10.3791/52799-v

•

11:24 min
June 17, 2015

•

8 Views
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1College of Physicians and Surgeons,Columbia University

Summary

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RNA in situ hybridization (ISH) enables the visualization of RNAs in cells and tissues. Here we show how combination of RNAscope ISH with immunohistochemistry or histological dyes can be successfully used to detect mRNAs localized to axons in sections of mouse and human brains.

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Baleriola, J., Jean, Y., Troy, C., Hengst, U. Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization. J. Vis. Exp. (100), e52799, doi:10.3791/52799 (2015).

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