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使用提顿体系的研究斑马鱼再生的分子机制
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration

使用提顿体系的研究斑马鱼再生的分子机制

DOI:

10:22 min

June 25, 2015

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Chapters

  • 00:05Title
  • 01:51Generation of TetActivator, TetResponder Double Transgenic Fish Lines
  • 03:51Tail Fin Amputation and Induction of Transgene Expression in the Regenerating Fin
  • 05:11Characterization of TetResponder Expression in Fin Regenerates
  • 08:08Results: Analysis of Fin Regeneration Using the TetOn System
  • 09:44Conclusion

Summary

自动翻译

Here we outline the workflow for using the TetON system to achieve tissue-specific gene expression in the adult regenerating zebrafish tail fin.

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