Journal
/
发育生物学
/
Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration

Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration

DOI:
10.3791/52756-v

•

10:22 min

•

June 25, 2015

•

, ,
1Institute for Biochemistry and Molecular Biology,Ulm University

Chapters

  • 00:05Title
  • 01:51Generation of TetActivator, TetResponder Double Transgenic Fish Lines
  • 03:51Tail Fin Amputation and Induction of Transgene Expression in the Regenerating Fin
  • 05:11Characterization of TetResponder Expression in Fin Regenerates
  • 08:08Results: Analysis of Fin Regeneration Using the TetOn System
  • 09:44Conclusion

Summary

自动翻译

自动翻译

Here we outline the workflow for using the TetON system to achieve tissue-specific gene expression in the adult regenerating zebrafish tail fin.

Tags

ZebrafishTetON SystemInducible Gene ExpressionTail Fin RegenerationMolecular MechanismsTransgenic LinesGene Expression Analysis

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration

Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System

Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration

Use of Immunolabeling to Analyze Stable, Dynamic, and Nascent Microtubules in the Zebrafish Embryo

Chemical Amputation and Regeneration of the Pharynx in the Planarian Schmidtea mediterranea

Methods for the Study of Regeneration in Stentor

Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development

Examining Muscle Regeneration in Zebrafish Models of Muscle Disease

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System

Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System

Read Article