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发育生物学
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Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings
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发育生物学
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JoVE 杂志 发育生物学
Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

DOI:
10.3791/52581-v

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14:40 min

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October 25, 2015

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, , , , , , ,
1Département de sciences cliniques,CHUV, Université de Montréal, St-Hyacinthe, QC, 2Département d’obstetriques et de gynécologie,CHU Ste-Justine Research Centre, Université de Montréal, 3Département de neurosciences,CHU Ste-Justine Centre de recherche, Université de Montréal, 4Centre de recherche en reproduction animale (CRRA),Université de Montréal, St-Hyacinthe, QC

Chapters

  • 00:00Title
  • 00:56Anesthesia and Preparation of the Ewe
  • 02:13Start of Surgery
  • 09:58Surgical Closure
  • 11:59Post Operative Period
  • 12:16Results
  • 13:41Conclusion

Summary

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The chronically instrumented non-anesthetized fetal sheep model is used to study human fetal development in health and disease, because it permits surgical placement and maintenance of catheters and electrodes, repetitive blood sampling, substance injection, recording of bioelectrical activity, and in vivo imaging. We describe the procedures required to establish this model.

Tags

Keywords: Fetal SheepChronic InstrumentationMultivariate RecordingsNon-anesthetizedFetal DevelopmentPathophysiologic StimuliGlucocorticoid TreatmentTherapeutic HypothermiaCatheter PlacementBioelectrical ActivityIn Vivo Organ Imaging

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