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Design, Surface Treatment, Cellular Plating, and Culturing of Modular Neuronal Networks Composed of Functionally Inter-connected Circuits
1Department of Neurobiology, George S. Wise Faculty of Life Sciences,Tel-Aviv University, 2Department of Neuroscience and Brain Technologies,Istituto Italiano di Tecnologia, 3School of Electrical Engineering,Tel-Aviv University, 4School of Physics and Astronomy,Tel-Aviv University, 5Department of Informatics, Bioengineering, Robotics and System Engineering,University of Genova
Chapters
- 00:05Title
- 01:18PDMS Chip Preparations
- 02:49Petri Dish and Cover Slip Preparation
- 04:50Preparing the Multi-Electrode Array
- 07:19Plating the Cells
- 08:17In vitro Neural Network Formation and Analysis
- 10:04Conclusion
This manuscript describes a protocol to grow in vitro modular networks consisting of spatially confined, functionally inter-connected neuronal circuits. A polymeric mask is used to pattern a protein layer to promote cellular adhesion over the culturing substrate. Plated neurons grow on coated areas establishing spontaneous connections and exhibiting electrophysiological activity.
