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फॉरवर्ड जेनेटिक्स पहचानें मैक्रोफेज का प्रयोग स्क्रीन<em> Toxoplasma gondii</em> जीन महत्वपूर्ण प्रतिरोध करने के लिए IFN-γ-निर्भर सेल स्वायत्त प्रतिरक्षण
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JoVE 杂志 免疫与感染
Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
DOI:

11:21 min

March 12, 2015

, , , , , , ,

Chapters

  • 00:05Title
  • 02:15Isolation of Parasite Mutants with a Fitness Defect Following Activation of Infected Macrophages
  • 06:03Evaluation of Mutants to Determine if the Defect is at the Level of Parasite Survival or Replication Following Activation of Infected Macrophages
  • 09:16Results: T. gondii Mutants with Fitness Defects are Identified by Phase Contrast and Immunofluorescence Microscopy
  • 10:48Conclusion

Summary

自动翻译

Forward genetics is a powerful approach to identify genes in intracellular pathogens important for resistance to cell autonomous immunity. The current approach uses innate immune cells, specifically macrophages, to identify novel Toxoplasma gondii genes important for immune evasion.

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